The increasing prevalence of multidrug-resistant and hypervirulent bacterial strains represents a growing global healthcare concern. However, early detection of pathogenic microbes allow for timely care of patients and the prevention of infectious strains proliferation. In the face of the current challenges in profiling bacterial infections, we are designing a fast and user-friendly detection assay using fluorogenic DNAzymes as the molecular probe. Our fluorogenic DNAzymes are single-stranded functionalized DNA capable of cleaving a fluorophore-quencher construct specifically in the presence of E. coli. Upon cleavage, the quencher can no longer suppress the fluorophore, resulting in intense fluorescence. This fluorescence intensity can be also used to quantify the amount of E. coli, and potentially achieve strain-specific recognition. Our novel approach to early pathogen detection technology can potentially enhance our ability to respond to disease outbreaks from infectious bacteria.
The Dry Lab will be supporting the Wet Lab as they use SELEX to find a DNAzyme for a highly pathogenic strain of C. Difficile. During the SELEX process, the Dry Lab will analyze deep sequencing data, monitoring sequence diversity as selection progresses to find possible homologies or motifs that are enriched through selection, using a clustering algorithm. After SELEX has narrowed the DNA library to a few potential DNAzymes, the Dry Lab will use a genetic algorithm as an in silico approach to find highly specific DNAzyme sequences. The affinities of the selected sequences will be measured by the Wet Lab, and high-affinity sequences will be preferentially crossed-over and mutated to create the next generation, in an iterative process that will produce higher-affinity DNAzymes.
The iGEM community outreach team is focused on spreading the interesting work being done in the world of synthetic biology, and more specifically by our research team, across the Hamilton community. We put an extra emphasis on trying to educate and empower students who may be interested in exploring our area of research as a means of achieving two central goals. First, we hope to inform the members of our community about the possible applications of synthetic biology on a larger scale in order to garner more support and interest for the field. Secondly, and perhaps more importantly, we also are striving to give back to the very community that supports our work by providing unique opportunities to a variety of students. Our attempts to achieve these goals have manifested themselves through hosting a series of programs, from workshops to speaker series, that give us the best opportunity to spread the work of iGEM McMaster.